A third polypeptide associated with heavy and light chain subunits of class I HLA antigens in immune interferon-treated human melanoma cells
✍ Scribed by Patrizio Giacomini; Adriano Aguzzi; Raffaele Tecce; Paul B. Fisher; Soldano Ferrone
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 709 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
A third polypeptide associated with heavy and light chain subunits of class I HLA antigens in immune interferon-treated human melanoma cells* Recombinant immune interferon (IFN-y) increases the synthesis, expression and shedding of class I HLA antigens by the cultured human melanoma cell line Colo 38. The magnitude of the IFN-y-induced changes in class I HLA antigens is dependent on both the dose and the time of exposure to IFN-y and is more pronounced on the heavy chain subunit than on PI-microglobulin (Pzm). In addition, a third, distinct polypeptide with a molecular mass of 14 kDa is present as a major component of the class I HLA molecular pool in IFN-y-treated melanoma cells. As IFN-y preferentially increases the synthesis of the heavy chain over that of P2m, the newly induced 14-kDa component appears to quantitatively replace Pzm. The stoichiometric relationship between the three molecules suggests that the IFN-y-induced 14-kDa component may be involved in the insertion of the heavy chain subunit into the plasma membrane.