A thin-layer chromatographic method for the quantitative determination of d-mannose, d-glucose and d-galactose in aqueous solution

✍ Scribed by Barry B. Pruden; Gilles Pineault; Hassan Loutfi

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 566 KB
• Volume: 115
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98950-2

✦ Synopsis

A method for the determinatioo of 5-mannose, D-gkcose and D-galactose is described for commerciaHy avaiIable @ass thin-layer plates. The silica ge1 GF layer on the p&es was activated by a combination of heating and treatment with sodium bistiphite and a citrate-phosphate buffer. Ptates were developed twice with an acetone-+butanot-water (53 :4U:7) mixture, with heat treatment between developments. Spots were colored by treatment with ceric sulphate and subsequent charring, and quantitative analysis was made with a densitometer_ The time required for analysis of one plate (5 samples) was 3 h, and standard errors of estimate were 2.8 %, 3.6 7; and 5.6 p0 far the three sugzm, respectively, for 10 replicates at levels of up to 20.3 ,o/r mannose, 8.0 g/!I gkcose and 4.5 g/i galactose.