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A thermostable phytase fromBacillussp. MD2: cloning, expression and high-level production inEscherichia coli

✍ Scribed by Thi Thuy Tran; Gashaw Mamo; Bo Mattiasson; Rajni Hatti-Kaul

Book ID
106309790
Publisher
Springer-Verlag
Year
2009
Tongue
English
Weight
572 KB
Volume
37
Category
Article
ISSN
1476-5535

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✍ Yukio Takii; Katsuya Daimon; Yuzuru Suzuki πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 856 KB

The gene coding for a thermostable exo-alpha-1,4-glucosidase (alpha-glucoside glucohydrolase: EC 3.2.1.20) of Bacillus stearothermophilus ATCC 12016 was cloned within a 2.8-kb AvaI fragment of DNA using the plasmid pUC19 as a vector and Escherichia coli JM109 as a host. E. coli with the hybrid plasm