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A thermodynamic study of magnesium(II) interactions with mono- and dinucleotides

✍ Scribed by H.H. Trimm; R.C. Patel

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
624 KB
Volume
35
Category
Article
ISSN
0020-1693

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✦ Synopsis

The equilibrium constants for the interaction between magnesium(U) and a number of mono-and dinucleotides (AMP, TMP, GMP, CMP, d-ApA, d-TpT, d-GpG, d-CpC, d-pTpT) have been determined accurately at 25.0 "C and an ionic strength of 0.2 M by a computerized, multiparamagnetic curve fitting analysis of pH titmtion curves. The data are consistent with inner sphere binding of magnesium(II) with the phosphate group. The presence of an additional base in those dinucleotides without a terminal phosphate causes a decrease in the magnesium(II) association constant.

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## Abstract A thermodynamic study on the interaction between magnesium ion and human growth hormone (hGH) was studied at 27Β°C in NaCl solution (50 mM) using different techniques. Two techniques of ionmetry using a Mg^2+^selective membrane electrode and isothermal titration calorimetry were applied