A theory of complex multiple equilibria. The effect of polyU concentration on the binding of deoxyadenosine

✍ Scribed by Helen Han; J. Michael Schurr

• Book ID: 101715309
• Publisher: Wiley (John Wiley & Sons)
• Year: 1974
• Tongue: English
• Weight: 460 KB
• Volume: 13
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1974.360130213

✦ Synopsis

Abstract

A grand‐partition function method employed previously for the study of cooperative binding of nucleosides to polynucleotides is generalized to include the effect of changes in the polymer concentration, as well as the effect of changes in the nucleoside concentration. This method is applied to compute isotherms for the binding of deoxyadenosine by polyuridylic acid at various total concentrations of polyuridylic acid. It is found that these isotherms are extremely insensitive to the polymer concentration, as commonly assumed heretofore, and as demonstrated experimentally (to some degree) by Davies and Davidson. However, the conclusion inferred by Davies and Davidson that the complexes must be unimolecular in polyuridylic acid is now seen to be clearly unwarranted, though it remains still a viable possibility, until data over a much wider range of polyuridylic acid concentrations become available.