A Theory Facilitating the Investigation of Sub-resolution Membrane Trafficking Using Total Internal Reflection Fluorescence Microscopy

A membrane-bound FRET-based caspase sens

A membrane-bound FRET-based caspase sensor for detection of apoptosis using fluorescence lifetime and total internal reflection microscopy

✍ Brigitte Angres; Heiko Steuer; Petra Weber; Michael Wagner; Herbert Schneckenbur 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 745 KB

## Abstract A caspase sensor based on Förster resonance energy transfer between fluorescent proteins is reported. Enhanced cyan fluorescent protein anchored to the inner leaflet of the plasma membrane of living cells is optically excited by an evanescent electromagnetic field and transfers its exci