The spontaneous temperature sensitive mutant 72c is shown to be more tolerant to fusidic acid, but less tolerant to trimethoprim on plates at permissive temperature, than is the parental strain. The poor growth of the mutant on amino acids supplemented plates, as well as its inability to grow on bro
A temperature-sensitive osmophilic mutant ofZygosaccharomyces rouxii
β Scribed by S. Yoshikawa; Y. Togawa; N. Mitsui; K. Chikara; G. Taguchi; M. Shimosaka; M. Okazaki
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 234 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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β¦ Synopsis
A temperature-sensitive osmophilic mutant of Zygosaccharomyces rouxii, OS15, was isolated, which required high salt or sugar concentration for growth above 30Β°C. Cell viability at 35"C in the presence of NaC1 was higher than in the absence of NaCI, and a survival ratio of the mutant cells after incubation at 55"C was also higher in the presence of NaCI than NaCl-free condition. Furthermore, resistance to UV light, hygromycin B and geneticin was improved in the presence of NaC1. There was no difference between the parent and the mutant in fatty acid saturation and microscopic cell shape under NaCI condition.
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A heat sensitive mutant of E. coli has been analyzed. A shift to restrictive temperature leads to an accumulation of ppGpp and pppGpp in both the parental and the mutant strains (both are relA+). The pool of these compounds is shown to decrease with time after the temperature shift in the case of th
## Abstract A temperatureβsensitive growth mutant derived from the BHK 21 cell Line, ts AF8, was found to have greatly reduced DNA synthesis at the nonpermissive temperature. This reduction is mainly due to a decrease in the frequency of cells synthesizing DNA. Upon shift up, ts AF8 becomes blocked