A technique for coating capillary columns with a very thick film of cross-linked stationary phase

✍ Scribed by Blomberg, S. ;Roeraade, J.

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 433 KB
• Volume: 11
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240110606

✦ Synopsis

A method for preparation of capillary columns and traps with a very thick film (up to 100 pm) of stationary phase is described. The principle of this method is based on an immediate fixation of a film of prepolymer, formed during dynamic coating. Thus, the development of film irregularities, such as are caused by Rayleigh instability i s avoided. Fixation of the film is conveniently accomplished by heat-accelerated crosslinking as was demonstrated in this work, where a commercially available silicone prepolymer (Sylgard 184, Dow Corning) was employed.

The low phase ratio columns which thus can be prepared are interesting both in chromatography with dense mobile phases and as enrichment devices. Examples of the latter application are shown, where trace organic components from air and water were concentrated.