of that nucleic acid sequence by conventional DNA-A quantitative hybridization technique for the debased assays.
tection of plasmid DNA by the action of a nuclease
Table 1 below summarizes the combinations of methenzyme is described. The process utilizes the specific ods that have been used to identify a target nucleic capture and detection of a sandwich hybridization, acid sequence. in a microtiter plate, that occurs in a single step. The Many of the recent developments reported have condetector probe is labeled with nuclease P 1 . The pHcentrated on the identification of PCR products (see dependent specificity of this enzyme for 3 -dinucleo-Refs. in Table 1) to enable the quantitation of the nutides is used to generate a measurable signal by cleic acid sequence in the original sample. These methactivating apo-glucose oxidase, which triggers an enods could also be applied to the direct detection of DNA zyme amplification cascade in the same microtiter in a sample in combination with a sensitive signalplate. The sensitivity of the assay system is demongenerating system, thereby circumventing the use of strated in an assay of a mutated form of the human PCR as a quantitative tool. Quantitation of DNA is pancreatic ribonuclease gene inserted into the plasmost amenable through capture of the target sequence mid pUC 18. The system was able to detect 35 amol on to a solid support followed by an appropriate detecof target DNA in an assay composed of a 60-min hytion reaction. The capture of a nucleic acid sequence bridization and 20 min of signal generation. This use of nuclease P 1 as the enzyme label and apo-glucose by allele-specific oligonucleotide probes would provide oxidase as the trigger for the amplification cascade the most specific and accurate diagnostic test. This ofresults in an assay that is more sensitive than prefers the potential to diagnose disease conditions much viously described enzyme amplification systems usearlier than current assays that rely on the detection ing colorimetric detection.