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A system for temperature-controlled expression of a foreign gene with dual mode in Saccharomyces cerevisiae

✍ Scribed by Hikari Kobayashi; Nobushige Nakazawa; Satoshi Harashima; Yasuji Oshima


Book ID
113396412
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
566 KB
Volume
69
Category
Article
ISSN
0922-338X

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A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and acti