A system for routine plantlet regeneration of sunflower (Helianthus annuusL.) from immature embryo-derived callus

✍ Scribed by Anne Wilcox McCann; Gloria Cooley; Jennifer Dreser

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 438 KB
• Volume: 14
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041183

✦ Synopsis

Immature embryos from inbred commercial cultivars of sunflower (Helianthus annuus L.) were used as donor material for induction of regenerable tissue in vitro. Optimum regeneration frequencies were obtained by transferring the tissue through a sequence of defined media using a specified timetable. The first medium was characterized by a high sucrose content (12%), 10#M 2,4-dichlorophenoxyacetic acid and 5#M abscisic acid. Within 7 days, proliferation of smooth, white, dedifferentiated tissue from the cotyledons was evident. After 3 weeks, the tissues were transferred through a series of 3 media, designed to promote shoot formation, shoot elongation and rooting. Regenerates were obtained with all 7 genotypes tested. From 1983 to 1984, approximately 500 primary R 0 plantlets were regenerated, grown to maturity in a greenhouse and self-pollinated. The resultant R~ seeds were subsequently field-grown and the plants were evaluated for variation.