A system for identification of triglycerides in reversed phase HPLC chromatograms based on equivalent carbon numbers
✍ Scribed by Podlaha, Oldrich ;Töregård, Bengt
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 402 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
A graphical system for tentative identification and prediction of peaks in reversed phase HPLC chromatograms of triglycerides is presented. Straight, parallel lines were found for different homologous series of triglycerides when plotting carbon numbers (CN) against equivalent chain numbers (ECN). ECN, defined as the carbon number of the hypothetical saturated triglyceride, which elutes at the same retention time as the unsaturated triglyceride being studied, are given for common triglycerides in vegetable oils for HPLC systems with propionitrile and acetonitrile/acetone (64/36) as mobile phases. The method is presumably also applicable to homologous series of other compounds such as wax esters.