𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A synthetic substrate assay for the gamma-secretase of the β-A4 amyloid of alzheimer's disease

✍ Scribed by Dr. Geneviève Evin; Konrad Beyreuther; Colin L. Masters

Book ID
105360086
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
600 KB
Volume
1
Category
Article
ISSN
1075-2617

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

γ‐secretase, the endoprotease which releases the C‐terminus of βA4 amyloid peptide, cleaves within the hydrophobic transmembrane domain of the amyloid precursor protein. In order to obtain a substrate for γ‐secretase, a dodecapeptide which spans the cleavage site was synthesized, labelled with 125‐iodine and conjugated to an agarose gel. A radiometric solid‐phase assay was developed using this immobilized substrate. Peptide products were separated by reverse‐phase HPLC and TLC to allow characterization of the cleavage site(s).

📜 SIMILAR VOLUMES

β-secretase as a target for the treatmen
β-secretase as a target for the treatment of Alzheimer's disease
✍ Martin Citron 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 490 KB 👁 1 views

## Abstract Finding inhibitors of the first step of the amyloid cascade, Aβ~42~ generation, is a major goal of Alzheimer's disease drug development. Two target protease activities, β‐and γ‐secretase, were detected more than 10 years ago but progress in this area has been slow because the enzymes we