✦ LIBER ✦

A surface plasmon resonance immunosensor based on the streptavidin-biotin complex

✍ Scribed by H. Morgan; D.M. Taylor

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 539 KB
Volume: 7
Category: Article
ISSN: 0956-5663
DOI: 10.1016/0956-5663(92)85039-d

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✦ Synopsis

It is shown that a streptavidin monolayer immobilized onto an evaporated gold film with biotin forms the basis of a highly specific sensing element. As an example, we show that by immobilizing the biotinylated antibody sex hormone binding globulin (alpha-SHBG) to the bound streptavidin monolayer a specific sensor for the antigen SHBG is readily fabricated. The interaction between immobilized antibody and corresponding antigen is monitored by surface plasmon resonance spectroscopy and is shown to follow a classic Langmuir isotherm. Detection of SHBG at nanomolar concentrations is demonstrated.

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