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A study of rose bengal and sulfhydryl reagents on stromal fragments of rabbit and human erythrocytes

✍ Scribed by Dastoli, Frank R. ;Green, James W.

Publisher: Wiley (John Wiley & Sons)
Year: 1965
Tongue: English
Weight: 469 KB
Volume: 65
Category: Article
ISSN: 0095-9898
DOI: 10.1002/jcp.1030650304

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✦ Synopsis

Stromal fragments of human and rabbit erythrocytes, prepared by osmotic hemolysis, were used as sources of ATPase i n a study of the action of the fluorescein dye, rose bengal, and of sulfhydryl reagents on this membrane component. Only the Na-K independent ATPase was systematically studied but the dye was observed, at concentrations of 10-3 M, to completely inhibit the Na-K dependent activity. Over a concentration range of 1.6 x 10-7 to 10-3 M the dye had a graded inhibitory effect on the Na-K independent ATPase in Tris buffer with little difference in the sensitivity of the enzyme from the stroma of the two species. The activity of the cation independent enzyme was not changed by darkness or anaerobic conditions in the presence of the dye and its action could not be attributed to photosensitization.

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