A study of apo- and holo-forms of horse liver alcohol dehydrogenase in solution by diffuse X-ray scattering
✍ Scribed by M. Yu. Pavlov; M. A. Sinev; A. A. Timchenko; O. B. Ptitsyn
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1986
- Tongue
- English
- Weight
- 607 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
of Sciences of the USSR, 142292 pushchino, Moscow Region, USSR
Synopsis
Apo-and holo-forms of horse liver alcohol dehydrogenase (LADH) in solution were studied by diffuse x-ray scattering. Experimental scattering curves for apo-and holoforms coincide both with the curves calculated from the crystal structures of apo-and holo-enzymes, and with each other. Thus the "sliding" of catalytical domains in LADH upon substrate binding, which has been shown by x-ray analysis, cannot he detected by diffuse x-ray scattering. Sensitivity of the scattering curves to the domain displacements of sliding and "locking" types has been investigated. i t has been shown that the scattering curves of LADH are rather sensitive to the domain "unlocking." However, these curves change only slightly upon sliding of domains, including the sliding of domains observed in LADH by x-ray analysis.