A structure based model for liposome disruption and the role of catalytic activity in myotoxic phospholipase A2s
β Scribed by M.T. Murakami; R.K. Arni
- Book ID
- 116921026
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 394 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0041-0101
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Secretory phospholipase A 2 (sPLA 2 ) belongs to a family of small interfacially active enzymes that catalyze the hydrolysis of phospholipids, yielding lysolipids and fatty acids. sPLA 2 is found in snake and bee venom and also in inflammatory and cancerous tissue. It is mainly active on organized l
The role of aspartic acid49 (Asp-49) in the active site of porcine pancreatic phospholipase A2 was studied by recombinant DNA techniques: two mutant proteins were constructed containing either glutamic acid (Glu) or lysine (Lys) at position 49. Enzymatic characterization indicated that the presence