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A streamlined method for rapid and sensitive chromatin immunoprecipitation

โœ Scribed by Michael L. Sikes; Justin M. Bradshaw; Wendell T. Ivory; Jessica L. Lunsford; Ruth E. McMillan; Clayton R. Morrison

Book ID
113717667
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
286 KB
Volume
344
Category
Article
ISSN
0022-1759

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โœ Kuo-Ping Huang; J.C. Robinson ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 421 KB

A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein