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A strategy for rapid and efficient DNA sequencing by mass spectrometry

✍ Scribed by Köster, Hubert; Tang, Kai; Fu, Dong-Jing; Braun, Andreas; van den Boom, Dirk; Smith, Cassandra L.; Cotter, Robert J.; Cantor, Charles R.

Book ID
109907889
Publisher
Nature Publishing Group
Year
1996
Tongue
English
Weight
754 KB
Volume
14
Category
Article
ISSN
1087-0156

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A modification procedure for Lys-C peptides is described which simplifies the correct assignment of the amino acid sequence. Release of the C-terminal lysine from Lys-C peptides by carboxypeptidase B and subsequent N-terminal acetylation of the resulting peptides leads to predictable shifts of the C