A strategy for mapping and neutralizing conformational immunogenic sites on protein therapeutics

Site-directed 13C solid-state NMR studie

Site-directed 13C solid-state NMR studies on membrane proteins: strategy and goals toward revealing conformation and dynamics as illustrated for bacteriorhodopsin labeled with [1-13C]amino acid residues

✍ Hazime Saitô; Jun Mikami; Satoru Yamaguchi; Michikazu Tanio; Atushi Kira; Tadash 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 419 KB

## Abstract We have so far demonstrated that well‐resolved and site‐specifically assigned ^13^C peaks as recorded by site‐directed NMR study on ^13^C‐labeled membrane proteins can serve as a convenient probe to reveal their local conformation and dynamics. We attempted here to clarify the extent to