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A Strategy for Identification of Oligosaccharide Structures Using Observational Multistage Mass Spectral Library

✍ Scribed by Kameyama, Akihiko; Kikuchi, Norihiro; Nakaya, Shuuichi; Ito, Hiromi; Sato, Takashi; Shikanai, Toshihide; Takahashi, Yoriko; Takahashi, Katsutoshi; Narimatsu, Hisashi


Book ID
118030169
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
255 KB
Volume
77
Category
Article
ISSN
0003-2700

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Oligosaccharides released from porcine thyroglobulin were first derivatised with 2-aminoacridone (2-AMAC) and analysed by capillary electrophoresis to determine the complexity of this glycan pool. The same glycan mixture was then subjected to either a sialidase digest or a sialidase and fucosidase d