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A stoichiometric model of Acidithiobacillus ferrooxidans ATCC 23270 for metabolic flux analysis

✍ Scribed by C. Hold; B.A. Andrews; J.A. Asenjo

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 185 KB
Volume: 102
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.22183

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✦ Synopsis

Abstract

A stoichiometric model of Acidithiobacillus ferrooxidans based on the sequenced genome from strain ATCC 23270 is derived and parameterized using genome/pathway databases. The model describes the main aspects of catabolism and anabolism. By the construction and utilization of the mathematical determination of the network, metabolic flux analysis is performed for such a bacterium for the first time and results are successfully verified by comparison to literature values. This first metabolic model of A. ferrooxidans is able to simulate the main aspects of metabolism and will be useful for further investigation and improvement of bioleaching procedures. Biotechnol. Bioeng. 2009;102: 1448–1459. © 2008 Wiley Periodicals, Inc.

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## Abstract Metabolic flux analysis (MFA) is widely used to quantify metabolic pathway activity. Typical applications involve isotopically labeled substrates, which require both metabolic and isotopic steady states for simplified data analysis. For bacterial systems, these steady states are readily