𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A stable isotope tracer method to measure silicic acid uptake by marine phytoplankton

✍ Scribed by David M. Nelson; John J. Goering

Book ID
102982609
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
569 KB
Volume
78
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A tracer method is described that uses the stable isotope %i to measure rates of silicic acid uptake by diatom cultures and natural populations of marine phytoplankton.

The method involves (i) incubation of organisms requiring silicic acid for growth in the presence of 30Si-labeled silicic acid, (ii) collection of the resulting particulate silicon, (iii) conversion of the particulate silicon to BaSiF,, (iv) determination of the 3oSi content of BaSiF, by solid sample mass spectrometry, and (v) calculation of the uptake rate from the YSi enrichment of the particulate matter during the incubation. The maximum overall error in the uptake rate measurement is f 10%.

📜 SIMILAR VOLUMES

A Liquid Chromatography–Mass Spectrometr
A Liquid Chromatography–Mass Spectrometry Method to Measure Stable Isotopic Tracer Enrichments of Glycerol and Glucose in Human Serum
✍ T.S. McIntosh; H.M. Davis; D.E. Matthews 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 120 KB

Stable isotopes are commonly used as tracers for the measurement of glycerol and glucose kinetics in metabolic studies. Traditionally, the analysis of these isotopes has been performed using gas chromatographymass spectrometry, which requires that the analytes first be derivatized. The derivatizatio

Sensitive determination of tyrosine meta
Sensitive determination of tyrosine metabolites, p-hydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenyl-acetic acid and 4-hydroxy-3-methoxymandelic acid, by gas chromatography—negative-ion chemical-ionization mass spectrometry : Application to a stable isotope-labelled tracer experiment to investigate their metabolism in man
✍ Michio Shimamura; Satoru Kamada; Tokishi Hayashi; Hiroshi Naruse; Yoshio Iida 📂 Article 📅 1986 🏛 Elsevier Science ⚖ 689 KB
Fortified Foods with Vitamins (Analytica
Fortified Foods with Vitamins (Analytical Concepts to Assure Better and Safer Products) || Quantitation of Pantothenic Acid in Fortified Foods by Stable Isotope Dilution Analysis and Method Comparison with a Microbiological Assay
✍ Rychlik, Michael 📂 Article 📅 2011 🏛 Wiley-VCH Verlag GmbH & Co. KGaA 🌐 German ⚖ 122 KB 👁 2 views

Unique in its review of modern analytical approaches to vitamin fortification, this book emphasizes fast, sensitive, and accurate methods, along with assays enabling the detection of various isomers and multiple vitamins. The expert contributors describe the concepts as well as analytical and assay