In an effort to understand more about the perturbing properties of adamantane-like molecules on biological membranes, the spin probe adamantyl nitroxide (2,2'-dimethyl-5-adamantyl oxazolidine-N-oxyl) was synthesized, purified and characterized. Electron paramagnetic resonance (EPR) spectra were then
A spin-labeled lipid for probing biological membranes
โ Scribed by A.S. Waggoner; T.J. Kingzett; S. Rottschaefer; O.H. Griffith; A.D. Keith
- Book ID
- 103039269
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 384 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0009-3084
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โฆ Synopsis
A spin-labeled hpld has been synthesized m ,~hmh the mtroxlde is rlgldl~ bound to a stearin acid chain at the 12-posmon The spin-labeled hpmd is sufficiently stable for general use m studying bmologmal membranes and model membrane systems Interactions of this new spin label with sodmm dodec~l sulfate and lecithin mlcellar solutions ha~e been m'~est~gated by electron spin resonance The electron spin resonance spectra of the solubdlzed spin labeled hpld are sensmve to changes in mlcellar structure v~hlch affect the mobd~ty of the label
๐ SIMILAR VOLUMES
Observations are made relating to the local environment and motional behavior of spin labeled lipids incorporated into human serum lipoproteins. Use has been made of ascorbic acid as a spin subtracting agent to determine the accessibility of the labeled site to the aqueous region.