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A spheroplast assay for recombination in bacteriophage lambda DNA

✍ Scribed by Gerald Cohen


Book ID
119008801
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
286 KB
Volume
58
Category
Article
ISSN
0042-6822

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The rate of formation of spheroplasts of yeast can be used as an assay to study the structural integrity of cell walls. Lysis can be measured spectrophotometrically in hypotonic solution in the presence of Zymolyase, a mixture of cell wall-digesting enzymes. The optical density of the cell suspensio