✦ LIBER ✦

A spectrophotometric assay for iodide oxidation by thyroid peroxidase

✍ Scribed by Nicholas M. Alexander

Publisher: Elsevier Science
Year: 1962
Tongue: English
Weight: 268 KB
Volume: 4
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(62)90097-0

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✦ Synopsis

Manometry

is useful, but inexpedient, for measuring thyroid peroxidase activity

(1) and recently the enzyme was assayed by a colorimet.ric guaiacol method (2). This report describes a rapid, sensitive spectrophotometric assay with the nat.ural substrate, iodide ion, rather than an artificial hydrogen donor.

Peroxidase catalyzes the formation of I, as depicted in Eq. ( 1) and periodide formation is then instantaneous (Eq. 2), when there is an iodide 2 KI + H?Oz peroxidasr I?+L)KOH IzfKI % KII (2)

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