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A specific, sensitive method for the determination of hyaluronate

✍ Scribed by George W. Jourdian; Melinda Wolfman; Robert Sarber; Jack Distler

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
573 KB
Volume
96
Category
Article
ISSN
0003-2697

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✦ Synopsis

A sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The P-A4,5-eneglucopyranuronic acid residues contained at the nonreducing termini of the resulting oligosaccharides were oxidized with periodic acid to yield, among other products, formyl pyruvic acid. The latter compound reacted with thiobarbituric acid to yield a chromophore with an absorption maximum at 549 nm. Optimal conditions for quantitative assay of hyaluronate are described.

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