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A specific inhibitor ofColletotrichum lindemuthianumprotease from kidney bean (Phaseolus vulgaris) seeds

✍ Scribed by V. V. Mosolov; M. D. Loginova; E. L. Malova; I. I. Benken

Book ID: 104750935
Publisher: Springer-Verlag
Year: 1979
Tongue: English
Weight: 454 KB
Volume: 144
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00388768

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✦ Synopsis

A specific protein an inhibitor of Colletotrichum lindemuthianum protease was isolated from kidney bean seeds in a homogeneous form. The purification procedure included gel filtration, isoelectric focusing and affinity chromatography on trypsin-Sepharose column. The latter was introduced to separate the fungal protease inhibitor from closely related trypsin and chymotrypsin inhibitors present in kidney bean seeds.

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