A software filter to remove interference ions from drug metabolites in accurate mass liquid chromatography/mass spectrometric analyses
✍ Scribed by Haiying Zhang; Donglu Zhang; Kenneth Ray
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 143 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1076-5174
- DOI
- 10.1002/jms.521
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✦ Synopsis
A software filter to remove interference ions from drug metabolites in accurate mass liquid chromatography/mass spectrometric analyses Liquid chromatography/mass spectrometric (LC/MS) identification of drug metabolites with low concentrations in biological fluids is always challenging due to significant interferences from endogenous species. Typically, the total ion chromatogram of an LC/MS experiment may show little resemblance to its corresponding metabolite profile, such as a radioactivity chromatogram; similarly, a metabolite ion signal in a mass spectrum may be embedded among those of irrelevant endogenous ions. A number of acquisition techniques, including parent scans, neutral loss scans, and MS/MS scans on predicted metabolite ions, have been utilized to identify metabolites in biological matrices. 1 Data-processing techniques have also been employed to sift through large amounts of LC/MS data to identify metabolite ions, examples of which include reconstituted ion chromatogram filtering for isotopic clusters and the use of