A site-directed antibody that inhibits phosphorylation of the rat-brain sodium channel by cyclic-AMP-dependent protein kinase
โ Scribed by Hitoshi Nakayama; Hiroyoshi Shikano; Yuichi Kanaoka
- Book ID
- 119121453
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 720 KB
- Volume
- 1175
- Category
- Article
- ISSN
- 0167-4889
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๐ SIMILAR VOLUMES
We investigated the effects of the purified catalytic subunit (C subunit) of the cAMP-dependent protein kinase (A-kinase) on the cardiac Na+ channel currents. Single Na+ channel currents in guinea-pig ventricular myocytes were recorded using the patch clamp technique of the inside-out configuration.
Both the neuronal IIA as well as the cardiac SkM2 isoform of the pore forming alpha-subunit of voltage dependent sodium channels are modulated by Protein Kinase A. While alphaIIA becomes attenuated upon PKA stimulation, alphaSkM2 becomes upregulated. PKC dependent phosphorylation of a serine, locate