A single nucleotide change in theMNR1 (VCX1/HUM1) gene determines resistance to manganese inSaccharomyces cerevisiae

✍ Scribed by Del Pozo, Lourdes; Osaba, Lourdes; Corchero, Javier; Jiménez, Antonio

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 172 KB
• Volume: 15
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(19990330)15:5<371::aid-yea380>3.0.co;2-p

✦ Synopsis

Several spontaneous Mn 2+ -resistant mutants were isolated from Saccharomyces cerevisiae strain W303-1b. All displayed an identical semidominant resistance phenotype. The gene responsible for this phenotype from one of these mutants, as well as that from the wild-type, was cloned and sequenced, which allowed the identification of a single nucleotide change in the former. Further sequencing work with the remaining mutants, as well as with others on the MNR1 gene , indicated that all displayed the same mutation (mnr1 allele). In addition, MNR1 was shown to correspond to VCX1 and HUM1, which determine a vacuolar membrane protein. The nucleotide sequence has been deposited at EMBL, with Accession No. AJ001272.