A simplified and reliable assay for the determination of erythrocyte pyridine nucleotide (NAD and NADP) concentrations, as well as the ratio of the reduced [NADH/NADPH] to oxidized \(\left[\mathbf{N A D}^{+} / \mathbf{N A D P}^{+}\right.\)] nucleotide, is important in understanding both normal and a
A single-extract method for the determination of oxidized and reduced diphosphopyridine nucleotide in rat liver
β Scribed by M.A. Spirtes; Herbert J. Eichel
- Book ID
- 118840292
- Publisher
- Elsevier Science
- Year
- 1954
- Tongue
- English
- Weight
- 276 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0003-9861
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π SIMILAR VOLUMES
Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio
An extraction procedure using mixtures of phenol, chloroform, and isoamyl alcohol originally applied to quench mitochondria for determining adenylates proved suitable also for the quantification of reduced and oxidized pyridine nucleotides yielding recoveries of more than 90%. In combination with HP