A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices
✍ Scribed by Zheyu Li; Kai Sun; Misato Sunayama; Ryoko Araki; Kosei Ueno; Masumi Abe; Hiroaki Misawa
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 249 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
Fraction collection of selected components from a complex mixture plays a critical role in biomedical research, environmental analysis, and biotechnology. Here, we introduce a novel electrophoretic chip device based on a signal processing theorem that allows simultaneous space sampling for fractionation of ssDNA target fragments. Ten parallel extraction channels, which covered 1.5‐mm‐long sampling ranges, were used to facilitate the capturing of fast‐moving fragments. Furthermore, the space sampling extraction made it possible to acquire pure collection, even from partly overlapping fragments that had been insufficiently separated after a short electrophoretic run. Fragments of 180, 181, and 182 bases were simultaneously collected, and then the recovered DNA was PCR amplified and assessed by CE analysis. The 181‐base target was shown to be isolated in a 70‐mm‐long separation length within 10 min, in contrast to the >50 min required for the 300‐mm‐long separation channel in our previous study. This method provides effective combination of time and space, which is a breakthrough in the traditional concept of fraction collection on a chip.