A simultaneous assay system for N-acetylgalactosamine and N-acetylgalactosaminitol using gas chromatography/mass spectrometry

✍ Scribed by Paul L. Weber; Don M. Carlson

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 404 KB
• Volume: 121
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90567-x

✦ Synopsis

A procedure is described, using gas chromatography/mass spectrometry to identify and quantitate various sugar alditol acetates, which will simultaneously quantitate N-acetylgalactosamine and N-acetylgalactosaminitol. N-acetylgalactosamine is distinguished from Nacetylgalactosaminitol by reduction with sodium borodeuteride and determining relative intensities of the ion pairs m/e 84, 85; 102, 103; and 144, 145. The method is adequate for determining amounts of these sugars in the range of 40-1000 nmol.