A simplified semiautomated assay for plasma triglycerides

✍ Scribed by Max E. Royer; Howard Ko

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 663 KB
• Volume: 29
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(69)90325-x

✦ Synopsis

Serum triglyceride levels which are higher than "normal" levels have been suggested as one of the biological indicat'ors of coronary artery disease (5). The semiautomated procedures of Lofland, Kessler, and Lederer, and Antonis (8-10) for measuring triglycerides require more or less extensive sample preparation. A new, simpler, extraction procedure has therefore been developed and the fluorometric procedure of Kessler and Lederer has been modified. MATERIALS Sodium methylate: 0.1 M sodium methylate (Matheson Coleman & Bell) in isopropanol. The isopropanol solubilizes the sodium alkoxide in the nonane sample stream during transesterification.

Sodium periodate (meta) : NaIO, (Mallinckrodt) ,0.025 M, dissolved in 5% acetic acid.

Acetylacetone reagent: 4oJ0, 2,4-pentanedione (puriss. grade, Aldrich Chemical Co.) in an aqueous solution of 2 M ammonium acetate (Mallinckrodt) . Use 1 hr after preparation and only for 2 days.

Nonane: distilled in vacua by Burdick and Jackson Laboratories,