A simplified radioactive assay for the enzyme UDP-glucose pyrophosphorylase

✍ Scribed by Christine A. Evers; Carl Mathew Palatnik

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 362 KB
• Volume: 118
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90164-0

✦ Synopsis

A radioactive assay for the enzyme UDP-glucose pyrophosphorylase has been modified. The m~ifications allow the enzyme to be assayed in one simple step and eliminate two procedures (a boiling step and an alkaline phosphatase incubation) which lengthen the time required to do the assay, make it somewhat cumbersome, and which must be carefully controlled. Furthermore, the modifications allow the simultaneous measurement of large numbers of samples. The modified assay is sensitive enough to quantitate activity in a mutant of Dictyostefium discoideum which with previous spectrophotometric assays could not be accurately measured.