A simplified protocol for the automated production of succinimidyl 4-[18F]fluorobenzoate on an IBA Synthera module
✍ Scribed by Uwe Ackermann; Shinn Dee Yeoh; John I. Sachinidis; Stan S. Poniger; Andrew M. Scott; Henri J. Tochon-Danguy
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- French
- Weight
- 237 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
The important peptide labelling reagent succinimidyl 4‐[^18^F]fluorobenzoate ([^18^F]SFB) has been synthesised in 75–85% decay corrected radiochemical yield using the IBA Synthera platform (IBA Cyclotron Solutions, Louvain‐la‐neuve, Belgium) with the fluorodeoxyglucose‐integrated fluidic processor nucleophilic and only four reagent vials in a single reactor. (4‐ethoxycarbonylphenyl) trimethylammonium triflate was used as the labelling precursor and 1 M aqueous tetramethylammonium hydroxide for the hydrolysis of the intermediate ethyl 4‐[^18^F]fluorobenzoate. N,N,N′,N′‐tetramethyl‐O‐(N‐succinimidyl)uronium tetrafluoroborate (TSTU) was then used to form [^18^F]SFB from 4‐[^18^F]fluorobenzoate. By omitting the addition of acetic acid and introducing a combined hydrolysis/water removal step, the synthesis time was shortened to 58 minutes. After SepPak purification, the radiochemical purity of [^18^F]SFB was 95.8–98.2%. These simplifications might be of significance to users of other automated synthesis modules.