A simplified process for the purification of recombinant nucleocapsid protein of nipah virus
โ Scribed by Fui Chin Chong; Wen Siang Tan; Dayang Radiah Awang Biak; Tau Chuan Ling; Beng Ti Tey
- Book ID
- 113682949
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 70 KB
- Volume
- 108
- Category
- Article
- ISSN
- 1389-1723
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## Abstract The nucleocapsid (N) protein of Nipah virus (NiV) can be produced in three __Escherichia coli__ strains [TOP10, BL21(DE3) and SG935] under the control of __trc__ promoter. However, most of the product existed in the form of insoluble inclusion bodies. There was no improvement in the sol
## Abstract A random peptide library of heptamers displayed on the surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with protein sequences of Nipah virus and several p