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A Simplified Procedure for a Rapid and Reliable Assay of both Glycogen and Trehalose in Whole Yeast Cells

✍ Scribed by Jean Luc Parrou; Jean François

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
77 KB
Volume
248
Category
Article
ISSN
0003-2697

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✦ Synopsis

An inherent disadvantage with the two-hybrid sys-A Simplified Procedure for a Rapid and Reliable tem, that is, preclusion of transactivating sequences Assay of both Glycogen and Trehalose in as bait, can be circumvented by incorporating a re-Whole Yeast Cells pressor domain in the bait hybrid protein. Repression provides a means to screen libraries using little or no 3-AT in the growth selection scheme and as a Jean Luc Parrou and Jean Franc ¸ois substitute to high concentrations of the inhibitor.

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