A Cell-Based Assay System for Monitoring
✍
Ki-Young Moon; Bum-Soo Hahn; Jinseon Lee; Yeong Shik Kim
📂
Article
📅
2001
🏛
Elsevier Science
🌐
English
⚖ 120 KB
A cell-based assay system for monitoring NF-kappaB activity was developed to determine the influence of activated NF-kappaB in human HaCaT cells. The pNF-kappaB-SEAP-NPT plasmid that permits expression of the secretory alkaline phosphatase (SEAP) reporter gene in response to the NF-kappaB activity a