A simplified method to determine binding parameters in a two-site case using linear regression

✍ Scribed by Vincenzo Guardabasso; Fabio Benfenati

• Book ID: 103053611
• Publisher: Elsevier Science
• Year: 1983
• Weight: 482 KB
• Volume: 17
• Category: Article
• ISSN: 0010-468X
• DOI: 10.1016/0010-468x(83)90044-2

✦ Synopsis

A program using a simplified method to compute estimates of binding parameters in a system with two independent classes of binding sites is presented. An iterative method is used to approximate step-by-step the two lines describing the binding activities separately, starting from a curvilinear Scatchard or Hofstee plot. This method is designed for use on microcomputers with graphic facilities and on programmable hand-held calculators.

Computer analysis of binding data Multiple binding sites Multiple receptors