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A simplified method for the simultaneous detection of intragenic and intergenic mutations (deletions) in Neurospora crassa

✍ Scribed by M.A. Luker; B.J. Kilbey


Book ID
113192327
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
278 KB
Volume
92
Category
Article
ISSN
0027-5107

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The 1.8-kb beta-globin gene fragments of DNAs from individuals heterozygous for nine different beta-thalassemia mutations involving 1, 2, 3, 4, or 25 basepair (bp) insertions or deletions were amplified by the polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on aqu