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A simplified analysis of scatchard plots for systems with two interacting binding sites

✍ Scribed by W. C. Galley; M. Bouvier; S.-D. Clas; G. R. Brown; L. E. St-Pierre

Publisher
Wiley (John Wiley & Sons)
Year
1988
Tongue
English
Weight
345 KB
Volume
27
Category
Article
ISSN
0006-3525

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✦ Synopsis

Synopsis

A simple graphical method for calculating stoichiometric and site binding constants for systems with two initially equivalent interacting sites is derived from a modified Scatchard equation. The binding constants can be calculated from Scatchard plots ( r / [ A ] as a function of r ) using the values of r / [ A ] ( r is the molar ratio of bound ligands to total protein and [ A ] is the equilibrium concentration of free ligand) when r = 0 and r = 1 (half-saturation). The applicability of the method to the adsorption of bilirubin by peptide pendants immobilized on a polyacrylamide support is demonstrated.

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