A simple stopped-flow temperature-jump apparatus

✍ Scribed by A.S. Verkman; James A. Dix; A.A. Pandiscio

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 430 KB
• Volume: 117
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90706-5

✦ Synopsis

A stopped-flow temperature-jump apparatus that has been used to extend the capabilities of a temperature-jump apparatus described previously (Verkman et al., Anal. Biochem. 102, 189-195, 1980) is described. The observation cell is a single cylinder made from ultraviolettransmitting Lucite. Stainless steel electrodes are fitted axially into each end of the cylinder, and solutions enter into and exit from the cylinder by passing through holes drilled perpendicular to the cylinder axis at the level of the electrodes. A triggering circuit provides a variable delay between solution mixing and the temperature-jump discharge. The mixing time for this instrument is under 1 ms, the dead time is under 60 ms, and the heating time is under 3 us. Solution reactions are followed by measuring optical absorption, fluorescence, or scattering with signal-to-noise ratios generally exceeding 5OO:l. The performance of this apparatus is illustrated by several membrane-substrate interactions.