A colour image processing algorithm is presented to identify and measure morphological features of mycelial aggregates in biomass stained with crystal violet to discriminate filamentous regions and compacted cores. The method improves the accuracy and allows automation of the previous monochrome pro
A simple staining procedure for the characterisation ofBasidiomycetespellets by image analysis
β Scribed by G. Durant; G. Crawley; P. Formisyn
- Book ID
- 104645691
- Publisher
- Springer-Verlag
- Year
- 1994
- Tongue
- English
- Weight
- 478 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
β¦ Synopsis
A simple staining procedure, associated with image analysis, was used to discriminate two zones (the filamentous zones and the compacted cores) in aggregates of fungal biomass according to their accessibility for an agent of the medium. The application of the method to batch fermentations of Schizophylfum commune showed a higher proportion of loosely entangled particles (clumps) and thinner filamentous outer zones obtained at a high agitation speed (500 rpm) compared to low agitation speeds (120-200 rpm).
π SIMILAR VOLUMES
A novel technique employed in preparing slides used for immunofluorescence staining of human tumor antigens with human anti-cancer monoclonal antibodies is described. The anchorage of the cancer cells to the plastic substratum is used as a basis in eliminating cell fixation by physical and chemical