A simple and rapid assay for measuring r
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William M. Mackin; Chi-Kuang Huang; Barbara-Jean Bormann; Elmer L. Becker
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Article
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1983
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Elsevier Science
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English
⚖ 716 KB
A simple, rapid assay for measuring radiolabeled ligand binding to purified plasma membranes was developed. In this assay, membrane proteins and ligand are mixed atop a nonmiscible silicone oil (density = 1.029 g/cm9 and incubated to establish equilibrium. The membrane proteins and bound ligand are