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A simple, rapid and inexpensive technique to bind small peptides to polystyrene surfaces for immunoenzymatic assays

✍ Scribed by Maria Antonietta Cuccuru; Daniele Dessì; Paola Rappelli; Pier Luigi Fiori


Book ID
113718110
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
195 KB
Volume
382
Category
Article
ISSN
0022-1759

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A simple, rapid assay for measuring radiolabeled ligand binding to purified plasma membranes was developed. In this assay, membrane proteins and ligand are mixed atop a nonmiscible silicone oil (density = 1.029 g/cm9 and incubated to establish equilibrium. The membrane proteins and bound ligand are