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A simple radioactivity assay for measurement of methionine adenosyltransferase activity by aqueous chromatography

✍ Scribed by Mehmet Öztürk; Frédérique Lemonnier; Danièle Cresteil; Alain Lemonnier

Book ID
117075401
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
319 KB
Volume
127
Category
Article
ISSN
0009-8981

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✍ L. Sankaran; Burton M. Pogell 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 937 KB

A simple, one-step procedure is described for measurement of the enzymatic transfer of radioactive methyl groups from S-adenosyl-r,-methionine to 0-demethylpuromycin. Reactions are carried out in small, stoppered scintillation vials and terminated by the addition of 0.1 M sodium borate-5 M NaCl (pH