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A simple modification on the vacuum system of the Beckman automated sequencer to improve the efficiency of edman degradation

✍ Scribed by Ajit S. Bhown; Thomas W. Cornelius; John E. Mole; J.Daniel Lynn; William A. Tidwell; J.Claude Bennett


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
238 KB
Volume
102
Category
Article
ISSN
0003-2697

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✦ Synopsis


A simple modification of the high-vacuum system of the Beckman automated sequencer is described. The air cylinder controlling the high vacuum to the reaction cup has been replaced with an electrically operated stainless-steel solenoid valve. The system maintains better high vacuum without the necessity for frequent oil changes. The efficiency has been demonstrated by the results of extended amino acid sequence analysis of various unknown proteins and peptides.