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A simple method to reduce the background and improve well-to-well reproducibility of staining in ELISPOT assays

โœ Scribed by Alexander Kalyuzhny; Sarah Stark


Book ID
114050842
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
214 KB
Volume
257
Category
Article
ISSN
0022-1759

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Protein kinases and phosphatases play an important role in a variety of cellular functions. Thus, it is of interest to develop an assay system that can be used to quantify the activity of individual enzymes specifically in a crude cellular extract, is simple to perform, and is amenable to automation