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A simple method to identify cysteine residues by isotopic labeling and ion trap mass spectrometry

✍ Scribed by Maciej Adamczyk; John C. Gebler; Jiang Wu

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
76 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

A simple method was developed to facilitate the identification of cysteine-containing peptides based on S-carboxymethylation of cysteine residues with isotopically enriched bromoacetic acid. An ion trap mass spectrometer with high-resolution scan functionality allows identification of the unique isotopic distribution patterns specific to the modified peptides.

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